cydB [2019-07-09 10:59:57]

cytochrome bd ubiquinol oxidase (subunit II), high affinity terminal oxidase

Locus: BSU38750

Isoelectric point: 9.48

Molecular weight: 37.70 kDa

Protein length: 338 aa

Gene length: 1017 bp

Function: respiration

Product: cytochrome bd ubiquinol oxidase (subunit II), high affinity terminal oxidase

Essential: no

Synonyms: yxkL

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Respiration → Terminal oxidases
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,976,791 → 3,977,807

The protein

Catalyzed reaction/ biological activity

• Ubiquinol-8 + O₂ = Ubiquinone-8 + H₂O (according to Swiss-Prot)

Cofactors

• heme b, heme d (according to UniProt)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: cydA-cydB-cydC-cydD
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon

  Regulatory mechanism

  • CcpA: repression, in CcpA regulon
  • Rex: repression, PubMed, in Rex regulon
  • ResD: activation, PubMed, in ResD regulon

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• MGNA-B746 (cydB::erm), available at the NBRP B. subtilis, Japan
• BKE38750 (ΔcydB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GATCATGAAGAGATGCCATG, downstream forward: _UP4_CATAAGGAGCCTATGACTTA
• BKK38750 (ΔcydB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GATCATGAAGAGATGCCATG, downstream forward: _UP4_CATAAGGAGCCTATGACTTA

References

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